en-usRapid Communications are short, expedited publications of research findings of exceptional importance. This manuscript type has been established to give authors the opportunity to submit in a concise format a novel research result for expedited assessment. This category is restricted to findings of sufficient scientific or clinical urgency to justify accelerated review and publication. Articles accepted for consideration in this category will be subject to accelerated handling, with a goal of time from initial submission to online publication of less than 90 days.Thu, 25 Apr 2024 08:08:17 GMThttp://cct.highwire.org/feeds/asn/rapid-communications.rss10.1681/ASN.2022050608Fri, 19 Aug 2022 10:51:51 GMT-07:00Netrin G1 Is a Novel Target Antigen in Primary Membranous NephropathyReinhard, LindaMachalitza, MayaWiech, ThorstenGröne, Hermann-JosefLassé, MoritzRinschen, Markus M.Ferru, NicolettaBräsen, Jan HinrichDrömann, FriederikeRob, Peter MariaSethi, SanjeevHoxha, ElionStahl, Rolf A.K.2022-08-19T10:51:51-07:00doi:10.1681/ASN.2022050608hwp:resource-id:jnephrol;33/10/1823American Society of NephrologyCopyright © 2022 by the American Society of NephrologyJournal of the American Society of Nephrologymembranous nephropathy, NTNG1 antibody, netrinsRapid CommunicationDialysisRapid CommunicationDialysisrapid-communication20222022-10-01October 202210.1681/ASN.20220506081046-66731533-34502022-08-19T10:51:51-07:002022-10Journal of the American Society of NephrologyRapid Communication3310101823i1831i10.1681/ASN.2022040504Tue, 16 Aug 2022 07:40:22 GMT-07:00SARS-CoV-2 Infection during the Omicron Surge among Patients Receiving Dialysis: The Role of Circulating Receptor-Binding Domain Antibodies and Vaccine DosesMontez-Rath, Maria E.Garcia, PabloHan, JialinCadden, LinaCelHunsader, PattiMorgan, CurtKerschmann, RussellBeyer, PaulDittrich, MaryBlock, Geoffrey A.Parsonnet, JulieChertow, Glenn M.Anand, Shuchi2022-08-16T07:40:22-07:00doi:10.1681/ASN.2022040504hwp:resource-id:jnephrol;33/10/1832American Society of NephrologyCopyright © 2022 by the American Society of NephrologyJournal of the American Society of Nephrologychronic dialysis, ESKD, immunology, clinical epidemiology, COVID-19, SARS-CoV-2Rapid CommunicationGlomerulonephritis and Interstitial NephritisRapid CommunicationGlomerulonephritis and Interstitial Nephritisresearch-article20222022-10-01October 202210.1681/ASN.20220405041046-66731533-34502022-08-16T07:40:22-07:002022-10Journal of the American Society of NephrologyRapid Communication33101832183910.1681/ASN.2021121578Fri, 22 Apr 2022 10:52:49 GMT-07:00Targeted Disruption of a Proximal Tubule–Specific TMEM174 Gene in Mice Causes Hyperphosphatemia and Vascular CalcificationMiyazaki-Anzai, ShinobuKeenan, Audrey L.Blaine, JudithMiyazaki, Makoto2022-04-22T10:52:49-07:00doi:10.1681/ASN.2021121578hwp:resource-id:jnephrol;33/8/1477American Society of NephrologyCopyright © 2022 by the American Society of NephrologyJournal of the American Society of NephrologyTMEM174, NPT2A, proximal tubule, hyperphosphatemia, vascular calcificationRapid CommunicationChronic Kidney DiseaseRapid CommunicationChronic Kidney Diseaseresearch-article20222022-08-01August 202210.1681/ASN.20211215781046-66731533-34502022-04-22T10:52:49-07:002022-08Journal of the American Society of NephrologyRapid Communication3381477148610.1681/ASN.2021121548Mon, 16 May 2022 01:17:09 GMT-07:00Arginase-1 Is Required for Macrophage-Mediated Renal Tubule RegenerationShin, Naomi S.Marlier, ArnaudXu, LeyuanDoilicho, NatnaelLinberg, DanielGuo, JiankanCantley, Lloyd G.2022-05-16T13:17:09-07:00doi:10.1681/ASN.2021121548hwp:resource-id:jnephrol;33/6/1077American Society of NephrologyCopyright © 2022 by the American Society of NephrologyJournal of the American Society of Nephrologykidney repair, macrophage, arginase-1, kidney tubules, regenerationRapid CommunicationAcute Kidney InjuryRapid CommunicationAcute Kidney Injuryresearch-article20222022-06-01June 202210.1681/ASN.20211215481046-66731533-34502022-05-16T13:17:09-07:002022-06Journal of the American Society of NephrologyRapid Communication3366611111077i1051212321241086i10532124212510.1681/ASN.2021101395Tue, 08 Feb 2022 08:27:07 GMT-08:00Comparative Effectiveness of mRNA-based BNT162b2 Vaccine versus Adenovirus Vector–Based Ad26.COV2.S Vaccine for the Prevention of COVID-19 among Dialysis PatientsBrunelli, Steven M.Sibbel, ScottKarpinski, StephMarlowe, GilbertWalker, Adam G.Giullian, JeffreyVan Wyck, DavidKelley, TaraLazar, RachaelZywno, Meredith L.Connaire, Jeffrey J.Young, AmyTentori, Francesca2022-02-08T08:27:07-08:00doi:10.1681/ASN.2021101395hwp:resource-id:jnephrol;33/4/688American Society of NephrologyCopyright © 2022 by the American Society of NephrologyJournal of the American Society of NephrologyCOVID-19, SARS-CoV-2, vaccine, dialysis, comparative effectiveness, BNT162 vaccineRapid CommunicationsRapid Communicationsresearch-article20222022-04-01April 202210.1681/ASN.20211013951046-66731533-34502022-02-08T08:27:07-08:002022-04Journal of the American Society of NephrologyRapid Communications33468869710.1681/ASN.2022010046Wed, 02 Feb 2022 09:35:54 GMT-08:00The Impact of Vaccination on Incidence and Outcomes of SARS-CoV-2 Infection in Patients with Kidney Failure in ScotlandBell, SamiraCampbell, JacquelineLambourg, EmilieWatters, ChrissieO’Neil, MartinAlmond, AlisonBuck, KatharineCarr, Edward J.Clark, LauraCousland, ZoeFindlay, MarkJoss, NicolaMetcalfe, WendyPetrie, MichaelaSpalding, ElaineTraynor, Jamie P.Sanu, VinodThomson, PeterMethven, ShonaMark, Patrick B.2022-02-02T09:35:54-08:00doi:10.1681/ASN.2022010046hwp:resource-id:jnephrol;33/4/677American Society of NephrologyCopyright © 2022 by the American Society of NephrologyJournal of the American Society of Nephrologyclinical epidemiology, vaccination, COVID-19, dialysis, transplantation, kidney replacement therapyRapid CommunicationsRapid Communicationsresearch-article20222022-04-01April 202210.1681/ASN.20220100461046-66731533-34502022-02-02T09:35:54-08:002022-04Journal of the American Society of NephrologyRapid Communications3333467767712281428143068612281430143110.1681/ASN.2021101323Tue, 11 Jan 2022 09:52:07 GMT-08:00Recurrence of Anti-Semaphorin 3B–Mediated Membranous Nephropathy after Kidney TransplantationFila, MarcDebiec, HannaPerrochia, HélèneDjouadi, NabilaVerpont, Marie-ChristineBuob, DavidRonco, Pierre2022-01-11T09:52:07-08:00doi:10.1681/ASN.2021101323hwp:resource-id:jnephrol;33/3/503American Society of NephrologyCopyright © 2022 by the American Society of NephrologyJournal of the American Society of Nephrologyglomerulopathy, immunology and pathology, membranous nephropathy, antibodies, semaphorin 3B, pediatric, kidney transplantation, recurrenceRapid CommunicationsRapid Communicationsresearch-article20222022-03-01March 202210.1681/ASN.20211013231046-66731533-34502022-01-11T09:52:07-08:002022-03Journal of the American Society of NephrologyRapid Communications33350350910.1681/ASN.2021081150Wed, 01 Dec 2021 09:28:12 GMT-08:00Spatially Resolved Transcriptomic Analysis of Acute Kidney Injury in a Female Murine ModelDixon, Eryn E.Wu, HaojiaMuto, YoshiharuWilson, Parker C.Humphreys, Benjamin D.2021-12-01T09:28:12-08:00doi:10.1681/ASN.2021081150hwp:resource-id:jnephrol;33/2/279American Society of NephrologyCopyright © 2022 by the American Society of NephrologyJournal of the American Society of Nephrologytranscriptomics, AKI, spatialRapid CommunicationsRapid Communicationsresearch-article20222022-02-01February 202210.1681/ASN.20210811501046-66731533-34502021-12-01T09:28:12-08:002022-02Journal of the American Society of NephrologyRapid Communications33227928910.1681/ASN.2021070998Wed, 10 Nov 2021 10:48:42 GMT-08:00Estimating Nephron Number from Biopsies: Impact on Clinical StudiesMorozov, DaryaParvin, NedaConaway, MarkOxley, GavinBaldelomar, Edwin J.Cwiek, AleksandradeRonde, KimBeeman, Scott C.Charlton, Jennifer R.Bennett, Kevin M.2021-11-10T10:48:42-08:00doi:10.1681/ASN.2021070998hwp:resource-id:jnephrol;33/1/39American Society of NephrologyCopyright © 2022 by the American Society of NephrologyJournal of the American Society of Nephrologynephron number, cationized ferritin-enhanced magnetic resonance imaging (CFE-MRI), biopsy, single nephron GFR (snGFR), virtual biopsy, glomerular densityRapid CommunicationsRapid Communicationsresearch-article20222022-01-01January 202210.1681/ASN.20210709981046-66731533-34502021-11-10T10:48:42-08:002022-01Journal of the American Society of NephrologyRapid Communications331394810.1681/ASN.2021060778Wed, 17 Nov 2021 10:26:37 GMT-08:00Real-World Effectiveness and Immunogenicity of BNT162b2 and mRNA-1273 SARS-CoV-2 Vaccines in Patients on HemodialysisSibbel, ScottMcKeon, KatherineLuo, JiacongWendt, KarlWalker, Adam G.Kelley, TaraLazar, RachaelZywno, Meredith L.Connaire, Jeffrey J.Tentori, FrancescaYoung, AmyBrunelli, Steven M.2021-11-17T10:26:37-08:00doi:10.1681/ASN.2021060778hwp:resource-id:jnephrol;33/1/49American Society of NephrologyCopyright © 2022 by the American Society of NephrologyJournal of the American Society of Nephrologyend stage kidney disease, end-stage renal disease, ESRD, dialysis, coronavirus disease 2019, COVID-19, SARS-CoV-2, vaccine, BNT162 vaccine, RNA, messengerRapid CommunicationsRapid Communicationsresearch-article20222022-01-01January 202210.1681/ASN.20210607781046-66731533-34502021-11-17T10:26:37-08:002022-01Journal of the American Society of NephrologyRapid Communications331495710.1681/ASN.2021070966Tue, 19 Oct 2021 05:27:18 GMT-07:00B and T Cell Responses after a Third Dose of SARS-CoV-2 Vaccine in Kidney Transplant RecipientsSchrezenmeier, EvaRincon-Arevalo, HectorStefanski, Ana-LuisaPotekhin, AlexanderStaub-Hohenbleicher, HenrietteChoi, MiraBachmann, FriederikeProβ, VanessaHammett, CharlotteSchrezenmeier, HubertLudwig, CarolinJahrsdörfer, BerndLino, Andreia C.Eckardt, Kai-UweKotsch, KatjaDörner, ThomasBudde, KlemensSattler, ArneHalleck, Fabian2021-10-19T05:27:18-07:00doi:10.1681/ASN.2021070966hwp:resource-id:jnephrol;32/12/3027American Society of NephrologyCopyright © 2021 by the American Society of NephrologyJournal of the American Society of NephrologyCOVID-19, vaccine, B cells, T cells, T-lymphocytes, SARS-CoV-2, kidney transplantationRapid CommunicationsRapid Communicationsresearch-article20212021-12-01December 202110.1681/ASN.20210709661046-66731533-34502021-10-19T05:27:18-07:002021-12Journal of the American Society of NephrologyRapid Communications32121244302729778698703033297886987110.1681/ASN.2021040432Wed, 04 Aug 2021 12:11:00 GMT-07:00Immunogenicity of SARS-CoV-2 Vaccine in DialysisLacson, EduardoArgyropoulos, Christos P.Manley, Harold J.Aweh, GideonChin, Andrew I.Salman, Loay H.Hsu, Caroline M.Johnson, Doug S.Weiner, Daniel E.2021-08-04T12:11:00-07:00doi:10.1681/ASN.2021040432hwp:resource-id:jnephrol;32/11/2735American Society of NephrologyCopyright © 2021 by the American Society of NephrologyJournal of the American Society of Nephrologydialysis, end-stage kidney disease, chronic kidney disease, vaccine, SARS-CoV-2, COVID-19, immune deficiency, clinical immunology, end-stage kidney diseaseRapid CommunicationsRapid Communicationsresearch-article20212021-11-01November 202110.1681/ASN.20210404321046-66731533-34502021-08-04T12:11:00-07:002021-11Journal of the American Society of NephrologyRapid Communications321127352742ckurts@web.de10.1681/ASN.2021010110Fri, 18 Jun 2021 11:02:42 GMT-07:00Renal Denervation Exacerbates LPS- and Antibody-induced Acute Kidney Injury, but Protects from Pyelonephritis in MiceBöhner, Alexander M.C.Jacob, Alice M.Heuser, ChristophStumpf, Natascha E.Effland, AlexanderAbdullah, ZeinabMeyer-Schwesiger, Catherinevon Vietinghoff, SibylleKurts, Christian2021-06-18T11:02:42-07:00doi:10.1681/ASN.2021010110hwp:resource-id:jnephrol;32/10/2445American Society of NephrologyCopyright © 2021 by the American Society of NephrologyJournal of the American Society of Nephrologyrenal denervation, chronic glomerulonephritis, pyelonephritis, sterile inflammation, septic kidney injury, neutrophilsRapid CommunicationsRapid Communicationsresearch-article20212021-10-01October 202110.1681/ASN.20210101101046-66731533-34502021-06-18T11:02:42-07:002021-10Journal of the American Society of NephrologyRapid Communications321010244523932453239510.1681/ASN.2021040480Thu, 10 Jun 2021 11:55:37 GMT-07:00Antibody and T Cell Response to SARS-CoV-2 Messenger RNA BNT162b2 Vaccine in Kidney Transplant Recipients and Hemodialysis PatientsBertrand, DominiqueHamzaoui, MouadLemée, VeroniqueLamulle, JulieHanoy, MélanieLaurent, CharlotteLebourg, LudivineEtienne, IsabelleLemoine, MathildeLe Roy, FrankNezam, DorianPlantier, Jean-ChristopheBoyer, OlivierGuerrot, DominiqueCandon, Sophie2021-06-10T11:55:37-07:00doi:10.1681/ASN.2021040480hwp:resource-id:jnephrol;32/9/2147American Society of NephrologyCopyright © 2021 by the American Society of NephrologyJournal of the American Society of Nephrologykidney transplantation, hemodialysis, clinical immunology, COVID-19Rapid CommunicationsRapid Communicationsresearch-article20212021-09-01September 202110.1681/ASN.20210404801046-66731533-34502021-06-10T11:55:37-07:002021-09Journal of the American Society of NephrologyRapid Communications3292147215210.1681/ASN.2021040490Wed, 16 Jun 2021 10:31:16 GMT-07:00Humoral Response after SARS-CoV-2 mRNA Vaccination in a Cohort of Hemodialysis Patients and Kidney Transplant RecipientsDanthu, ClémentHantz, SébastienDahlem, ArthurDuval, MarionBa, BacaryGuibbert, ManonEl Ouafi, ZhourPonsard, SéverineBerrahal, InsafAchard, Jean-MichelBocquentin, FrédériqueAllot, VincentRerolle, Jean-PhilippeAlain, SophieTouré, Fatouma2021-06-16T10:31:16-07:00doi:10.1681/ASN.2021040490hwp:resource-id:jnephrol;32/9/2153American Society of NephrologyCopyright © 2021 by the American Society of NephrologyJournal of the American Society of Nephrologyvaccine, humoral response, kinetic, hemodialyzed, kidney transplant recipients, COVID-19Rapid CommunicationsRapid Communicationsresearch-article20212021-09-01September 202110.1681/ASN.20210404901046-66731533-34502021-06-16T10:31:16-07:002021-09Journal of the American Society of NephrologyRapid Communications3292153215810.1681/ASN.2021030387Fri, 02 Jul 2021 08:30:07 GMT-07:00Antibody Status, Disease History, and Incidence of SARS-CoV-2 Infection Among Patients on Chronic DialysisCohen, Dena E.Sibbel, ScottMarlowe, GilbertBludorn, KelseyMiller, DawnKelley, TaraConnaire, JeffreyYoung, AmyTentori, FrancescaBrunelli, Steven M.2021-07-02T08:30:07-07:00doi:10.1681/ASN.2021030387hwp:resource-id:jnephrol;32/8/1880American Society of NephrologyCopyright © 2021 by the American Society of NephrologyJournal of the American Society of NephrologyCOVID-19, hemodialysis, immunityRapid CommunicationsRapid Communicationsresearch-article20212021-08-01August 202110.1681/ASN.20210303871046-66731533-34502021-07-02T08:30:07-07:002021-08Journal of the American Society of NephrologyRapid Communications3281880188610.1681/ASN.2020121762Fri, 28 May 2021 10:31:36 GMT-07:00A Biallelic Frameshift Mutation in Nephronectin Causes Bilateral Renal Agenesis in HumansDai, LeiLi, JingzhiXie, LiangqunWang, WeinanLu, YangXie, MingkunHuang, JingruiShen, KuifangYang, HuiPei, ChenlinZhao, YanhuaZhang, Weishe2021-05-28T10:31:36-07:00doi:10.1681/ASN.2020121762hwp:resource-id:jnephrol;32/8/1871American Society of NephrologyCopyright © 2021 by the American Society of NephrologyJournal of the American Society of Nephrologybilateral renal agenesis, new pathogenic gene, nephronectinRapid CommunicationsRapid Communicationsresearch-article20212021-08-01August 202110.1681/ASN.20201217621046-66731533-34502021-05-28T10:31:36-07:002021-08Journal of the American Society of NephrologyRapid Communications3281871187910.1681/ASN.2021010104Thu, 29 Apr 2021 04:53:52 GMT-07:00SARS-CoV-2 Vaccine Acceptability in Patients on Hemodialysis: A Nationwide SurveyGarcia, PabloMontez-Rath, Maria E.Moore, HeatherFlotte, JohnieFults, ChrisBlock, Martha S.Han, JialinDittrich, MaryParsonnet, JulieChertow, Glenn M.Block, Geoffrey A.Anand, Shuchi2021-04-29T04:53:52-07:00doi:10.1681/ASN.2021010104hwp:resource-id:jnephrol;32/7/1575American Society of NephrologyCopyright © 2021 by the American Society of NephrologyJournal of the American Society of Nephrologydialysis, vaccine, SARS-CoV-2, ESKD, COVID-19Rapid CommunicationRapid Communicationresearch-article20212021-07-01July 202110.1681/ASN.20210101041046-66731533-34502021-04-29T04:53:52-07:002021-07Journal of the American Society of NephrologyRapid Communication3271575158110.1681/ASN.2020111618Fri, 26 Feb 2021 10:17:37 GMT-08:00Kinetics of Anti–SARS-CoV-2 IgG Antibodies in Hemodialysis Patients Six Months after InfectionSakhi, HamzaDahmane, DjamalAttias, PhilippeKofman, ThomasBouvier, MagaliLapidus, NathanaelFourati, SlimEl Karoui, Khalil,,Audard, VincentBentaarit, BoutheinaBoueilh, AnnaGallien, SébastienGrimbert, PhilippeHüe, SophieJoher, NizarJouan, NarindraLamriben, LarbiLelièvre, Jean-DanielLepeule, RaphaëlMahévas, MatthieuMatignon, MarieMelica, GiovannaOniszczuk, JuliePawlotsky, Jean-MichelStehlé, ThomasVindrios, WilliamWemmert, Charlotte2021-02-26T10:17:37-08:00doi:10.1681/ASN.2020111618hwp:resource-id:jnephrol;32/5/1033American Society of NephrologyCopyright © 2021 by the American Society of Nephrology This is an Open Access article: American Society of NephrologyJournal of the American Society of Nephrologyhemodialysis, SARS-CoV-2, serology, SARS-CoV-2 antibody, COVID-19Rapid CommunicationRapid Communicationresearch-article20212021-05-03May 202110.1681/ASN.20201116181046-66731533-34502021-02-26T10:17:37-08:002021-05-03Journal of the American Society of NephrologyRapid Communication32551033i1036i10.1681/ASN.2020101537Mon, 01 Feb 2021 07:16:13 GMT-08:00A Novel Soluble ACE2 Variant with Prolonged Duration of Action Neutralizes SARS-CoV-2 Infection in Human Kidney OrganoidsWysocki, JanYe, MinghaoHassler, LuiseGupta, Ashwani KumarWang, YuguoNicoleascu, VladRandall, GlennWertheim, Jason A.Batlle, Daniel2021-02-01T07:16:13-08:00doi:10.1681/ASN.2020101537hwp:resource-id:jnephrol;32/4/795American Society of NephrologyCopyright © 2021 by the American Society of NephrologyJournal of the American Society of NephrologyACE2, SARS-CoV-2, soluble ACE2 proteins, ACE2 1-618-ABD, COVID-19, organoids, ACE2 decoyRapid CommunicationsRapid Communicationsother20212021-04-01April 202110.1681/ASN.20201015371046-66731533-34502021-02-01T07:16:13-08:002021-04Journal of the American Society of NephrologyRapid Communications32479580310.1681/ASN.2020060823Tue, 16 Feb 2021 08:29:16 GMT-08:00Interaction between GALNT12 and C1GALT1 Associates with Galactose-Deficient IgA1 and IgA NephropathyWang, Yan-NaZhou, Xu-JieChen, PeiYu, Gui-ZhenZhang, XueHou, PingLiu, Li-JunShi, Su-FangLv, Ji-ChengZhang, Hong2021-02-16T08:29:16-08:00doi:10.1681/ASN.2020060823hwp:resource-id:jnephrol;32/3/545American Society of NephrologyCopyright © 2021 by the American Society of NephrologyJournal of the American Society of Nephrologyhuman genetics, IgA nephropathy, glomerulonephritis, IgA, immune complexesRapid CommunicationsRapid Communicationsother20212021-03-01March 202110.1681/ASN.20200608231046-66731533-34502021-02-16T08:29:16-08:002021-03Journal of the American Society of NephrologyRapid Communications32354555210.1681/ASN.2020050717Fri, 29 Jan 2021 09:37:00 GMT-08:00Endothelial-Derived miR-17∼92 Promotes Angiogenesis to Protect against Renal Ischemia-Reperfusion InjuryChiba, TakutoCerqueira, Débora M.Li, YaoBodnar, Andrew J.Mukherjee, ElinaPfister, KatherinePhua, Yu LengShaikh, KaiSanders, Brandon T.Hemker, Shelby L.Pagano, Patrick J.Wu, Yijen L.Ho, JacquelineSims-Lucas, Sunder2021-01-29T09:37:00-08:00doi:10.1681/ASN.2020050717hwp:resource-id:jnephrol;32/3/553American Society of NephrologyCopyright © 2021 by the American Society of NephrologyJournal of the American Society of NephrologymicroRNA, acute kidney injury, renal microvasculatureRapid CommunicationsRapid Communicationsresearch-article20212021-03-01March 202110.1681/ASN.20200507171046-66731533-34502021-01-29T09:37:00-08:002021-03Journal of the American Society of NephrologyRapid Communications32355356210.1681/ASN.2020060804Thu, 19 Nov 2020 06:52:32 GMT-08:00COVID-19–Associated Glomerular DiseaseShetty, Aneesha A.Tawhari, IbrahimSafar-Boueri, LuisaSeif, NayAlahmadi, AmeenGargiulo, RichardAggarwal, VikramUsman, IrtazaKisselev, SergeyGharavi, Ali G.Kanwar, YahspalQuaggin, Susan E.2020-11-19T06:52:32-08:00doi:10.1681/ASN.2020060804hwp:resource-id:jnephrol;32/1/33American Society of NephrologyCopyright © 2021 by the American Society of NephrologyJournal of the American Society of NephrologyCOVID-19, collapsing glomerulopathy, APOL1Rapid CommunicationRapid Communicationresearch-article20212021-01-01Month XX, 202010.1681/ASN.20200608041046-66731533-34502020-11-19T06:52:32-08:002021-01Journal of the American Society of NephrologyRapid Communication321133140210.1681/ASN.2019101075Fri, 21 Aug 2020 07:56:25 GMT-07:00Differentiation of a Contractile, Ureter-Like Tissue, from Embryonic Stem Cell–Derived Ureteric Bud and Ex Fetu MesenchymeSallam, MayPalakkan, Anwar A.Mills, Christopher G.Tarnick, JuliaElhendawi, MonaMarson, LornaDavies, Jamie A.2020-08-21T07:56:25-07:00doi:10.1681/ASN.2019101075hwp:resource-id:jnephrol;31/10/2253American Society of NephrologyCopyright © 2020 by the American Society of NephrologyJournal of the American Society of Nephrologystem cell, renal stem cell, ureteric bud, kidney developmentRapid CommunicationRapid Communicationresearch-article20202020-10-01October 202010.1681/ASN.20191010751046-66731533-34502020-08-21T07:56:25-07:002020-10Journal of the American Society of NephrologyRapid Communication311010722532231i22622232i10.1681/ASN.2020060802Fri, 17 Jul 2020 08:06:30 GMT-07:00Kidney Biopsy Findings in Patients with COVID-19Kudose, SatoruBatal, IbrahimSantoriello, DominickXu, KatherineBarasch, JonathanPeleg, YonatanCanetta, PietroRatner, Lloyd E.Marasa, MaddalenaGharavi, Ali G.Stokes, M. BarryMarkowitz, Glen S.D’Agati, Vivette D.2020-07-17T08:06:30-07:00doi:10.1681/ASN.2020060802hwp:resource-id:jnephrol;31/9/1959American Society of NephrologyCopyright © 2020 by the American Society of NephrologyJournal of the American Society of Nephrologykidney biopsy, renal pathology, COVID-19Rapid CommunicationRapid Communicationresearch-article20202020-09-01September 202010.1681/ASN.20200608021046-66731533-34502020-07-17T08:06:30-07:002020-09Journal of the American Society of NephrologyRapid Communication3191959196810.1681/ASN.2020060827Thu, 30 Jul 2020 11:56:33 GMT-07:00High Prevalence of Asymptomatic COVID-19 Infection in Hemodialysis Patients Detected Using Serologic ScreeningClarke, CandicePrendecki, MariaDhutia, AmritaAli, Mahrukh A.Sajjad, HiraShivakumar, OshiniLightstone, LizKelleher, PeterPickering, Matthew C.Thomas, DavidCharif, RawyaGriffith, MeganMcAdoo, Stephen P.Willicombe, Michelle2020-07-30T11:56:33-07:00doi:10.1681/ASN.2020060827hwp:resource-id:jnephrol;31/9/1969American Society of NephrologyCopyright © 2020 by the American Society of NephrologyJournal of the American Society of Nephrologydialysis, serology, COVID-19Rapid CommunicationRapid Communicationresearch-article20202020-09-01September 202010.1681/ASN.20200608271046-66731533-34502020-07-30T11:56:33-07:002020-09Journal of the American Society of NephrologyRapid Communication3191969197510.1681/ASN.2020050699Mon, 13 Jul 2020 11:02:23 GMT-07:00COVID-19–Associated Kidney Injury: A Case Series of Kidney Biopsy FindingsSharma, PurvaUppal, Nupur N.Wanchoo, RimdaShah, Hitesh H.Yang, YiheParikh, RushangKhanin, YuriyMadireddy, VarunLarsen, Christopher P.Jhaveri, Kenar D.Bijol, Vanesa,Abate, MersemaPaz Andrade, HugoBarnett, Richard L.Bellucci, AlessandroBhaskaran, Madhu C.Corona, Antonio G.Flores Chang, Bessy SuyinFinger, MarkFishbane, StevenGitman, MichaelHalinski, CandiceHasan, ShamirHazzan, Azzour D.Hirsch, Jamie S.Hong, SusanaJhaveri, Kenar D.Khanin, YuriyKuan, AireenMadireddy, VarunMalieckal, DeepaMuzib, AbdulrahmanNair, GayatriNair, Vinay V.Ng, Jia H.Parikh, RushangRoss, Daniel W.Sakhiya, VipulbhaiSachdeva, MalaSchwarz, RichardShah, Hitesh H.Sharma, PurvaSinghal, Pravin C.Uppal, Nupur N.Wanchoo, Rimda2020-07-13T11:02:23-07:00doi:10.1681/ASN.2020050699hwp:resource-id:jnephrol;31/9/1948American Society of NephrologyCopyright © 2020 by the American Society of NephrologyJournal of the American Society of Nephrologykidney pathology, kidney biopsy, acute kidney injury, AKI, ATN, COVID-19, SARS-CoV-2Rapid CommunicationRapid Communicationresearch-article20202020-09-01September 202010.1681/ASN.20200506991046-66731533-34502020-07-13T11:02:23-07:002020-09Journal of the American Society of NephrologyRapid Communication3191948195810.1681/ASN.2020040432Tue, 05 May 2020 05:45:03 GMT-07:00Ultrastructural Evidence for Direct Renal Infection with SARS-CoV-2Farkash, Evan A.Wilson, Allecia M.Jentzen, Jeffrey M.2020-05-05T05:45:03-07:00doi:10.1681/ASN.2020040432hwp:resource-id:jnephrol;31/8/1683American Society of NephrologyCopyright © 2020 by the American Society of NephrologyJournal of the American Society of NephrologyCOVID-19, SARS-CoV-2, acute kidney failure, autopsy, renal pathology, electron microscopyRapid CommunicationRapid Communicationresearch-article20202020-08-01August 202010.1681/ASN.20200404321046-66731533-34502020-05-05T05:45:03-07:002020-08Journal of the American Society of NephrologyRapid Communication3131810168324941687249410.1681/ASN.2020050558Fri, 19 Jun 2020 10:06:04 GMT-07:00AKI and Collapsing Glomerulopathy Associated with COVID-19 and APOL1 High-Risk GenotypeWu, HuijuanLarsen, Christopher P.Hernandez-Arroyo, Cesar F.Mohamed, Muner M.B.Caza, TiffanySharshir, Moh’dChughtai, AsimXie, LipingGimenez, Juan M.Sandow, Tyler A.Lusco, Mark A.Yang, HaichunAcheampong, EllenRosales, Ivy A.Colvin, Robert B.Fogo, Agnes B.Velez, Juan Carlos Q.2020-06-19T10:06:04-07:00doi:10.1681/ASN.2020050558hwp:resource-id:jnephrol;31/8/1688American Society of NephrologyCopyright © 2020 by the American Society of NephrologyJournal of the American Society of Nephrologycollapsing glomerulopathy, APOL1, kidney biopsy, SARS-CoV-2, nephrotic, COVID-19Rapid CommunicationRapid Communicationresearch-article20202020-08-01August 202010.1681/ASN.20200505581046-66731533-34502020-06-19T10:06:04-07:002020-08Journal of the American Society of NephrologyRapid Communication3181688169510.1681/ASN.2020040470Thu, 28 May 2020 12:40:01 GMT-07:00Presentation and Outcomes of Patients with ESKD and COVID-19Valeri, Anthony M.Robbins-Juarez, Shelief Y.Stevens, Jacob S.Ahn, WooinRao, Maya K.Radhakrishnan, JaiGharavi, Ali G.Mohan, SumitHusain, S. Ali2020-05-28T12:40:01-07:00doi:10.1681/ASN.2020040470hwp:resource-id:jnephrol;31/7/1409American Society of NephrologyCopyright © 2020 by the American Society of NephrologyJournal of the American Society of Nephrologycoronavirus, dialysis, Epidemiology and outcomes, ESRD, COVID-19Rapid CommunicationRapid Communicationresearch-article20202020-07-01July 202010.1681/ASN.20200404701046-66731533-34502020-05-28T12:40:01-07:002020-07Journal of the American Society of NephrologyRapid Communication3171409141510.1681/ASN.2020030276Tue, 28 Apr 2020 02:53:06 GMT-07:00Renal Involvement and Early Prognosis in Patients with COVID-19 PneumoniaPei, GuangchangZhang, ZhiguoPeng, JingLiu, LiuZhang, ChunxiuYu, ChongMa, ZufuHuang, YiLiu, WeiYao, YingZeng, RuiXu, Gang2020-04-28T14:53:06-07:00doi:10.1681/ASN.2020030276hwp:resource-id:jnephrol;31/6/1157American Society of NephrologyCopyright © 2020 by the American Society of NephrologyJournal of the American Society of NephrologyPneumonia, proteinuria, Hematuria, Acute Kidney Failure, COVID-19Rapid CommunicationRapid Communicationresearch-article20202020-06-01June 202010.1681/ASN.20200302761046-66731533-34502020-04-28T14:53:06-07:002020-06Journal of the American Society of NephrologyRapid Communication3161157116510.1681/ASN.2020030375Tue, 21 Apr 2020 04:45:09 GMT-07:00Early Description of Coronavirus 2019 Disease in Kidney Transplant Recipients in New YorkThe Columbia University Kidney Transplant ProgramHusain, S. AliChang, Jae H.Cohen, David J.Crew, R. JohnDube, Geoffrey K.Fernandez, Hilda E.Morris, Heather K.Aaron, Justin G.Miko, Benjamin A.Pereira, Marcus R.Hardy, Mark A.McCune, Kasi R.Ratner, Lloyd E.Sandoval, P. RodrigoWeiner, JoshuaTsapepas, DemetraMohan, Sumit2020-04-21T16:45:09-07:00doi:10.1681/ASN.2020030375hwp:resource-id:jnephrol;31/6/1150American Society of NephrologyCopyright © 2020 by the American Society of NephrologyJournal of the American Society of Nephrologytransplant outcomes, kidney transplantation, Epidemiology and outcomes, COVID-19Rapid CommunicationRapid Communicationresearch-article20202020-06-01June 202010.1681/ASN.20200303751046-66731533-34502020-04-21T16:45:09-07:002020-06Journal of the American Society of NephrologyRapid Communication3166115011471156114910.1681/ASN.2019060573Thu, 30 Apr 2020 10:00:29 GMT-07:00In Vivo Assessment of Size-Selective Glomerular Sieving in Transplanted Human Induced Pluripotent Stem Cell–Derived Kidney Organoidsvan den Berg, Cathelijne W.Koudijs, AngelaRitsma, LailaRabelink, Ton J.2020-04-30T10:00:29-07:00doi:10.1681/ASN.2019060573hwp:resource-id:jnephrol;31/5/921American Society of NephrologyCopyright © 2020 by the American Society of NephrologyJournal of the American Society of Nephrologypluripotent stem cells, kidney organoids, kidney transplantation, intravital microscopy, glomerular filtrationRapid CommunicationRapid Communicationresearch-article20202020-05-01May 202010.1681/ASN.20190605731046-66731533-34502020-04-30T10:00:29-07:002020-05Journal of the American Society of NephrologyRapid Communication31592192910.1681/ASN.2019030303Fri, 06 Sep 2019 08:28:26 GMT-07:00A Toolbox to Characterize Human Induced Pluripotent Stem Cell–Derived Kidney Cell Types and OrganoidsVanslambrouck, Jessica M.Wilson, Sean B.Tan, Ker SinSoo, Joanne Y.-C.Scurr, MichelleSpijker, H. SiebeStarks, Lakshi T.Neilson, AmberCui, XiaoxiaJain, SanjayLittle, Melissa HelenHowden, Sara E.2019-09-06T08:28:26-07:00doi:10.1681/ASN.2019030303hwp:resource-id:jnephrol;30/10/1811American Society of NephrologyCopyright © 2019 by the American Society of NephrologyJournal of the American Society of Nephrologystem cell, kidney development, molecular biologyRapid CommunicationRapid Communicationresearch-article20192019-10-01October 201910.1681/ASN.20190303031046-66731533-34502019-09-06T08:28:26-07:002019-10Journal of the American Society of NephrologyRapid Communication30101811182310.1681/ASN.2018090912Mon, 03 Dec 2018 07:33:15 GMT-08:00Advantages of Single-Nucleus over Single-Cell RNA Sequencing of Adult Kidney: Rare Cell Types and Novel Cell States Revealed in FibrosisWu, HaojiaKirita, YuheiDonnelly, Erinn L.Humphreys, Benjamin D.2018-12-03T07:33:15-08:00doi:10.1681/ASN.2018090912hwp:resource-id:jnephrol;30/1/23American Society of NephrologyCopyright © 2019 by the American Society of NephrologyJournal of the American Society of NephrologyRNA-sequencing, fibrosis, single cellRapid CommunicationRapid Communicationresearch-article20192019-01-01January 201910.1681/ASN.20180909121046-66731533-34502018-12-03T07:33:15-08:002019-01Journal of the American Society of NephrologyRapid Communication301233210.1681/ASN.2018050519Tue, 02 Oct 2018 07:26:26 GMT-07:00Inhibitory Anti-Peroxidasin Antibodies in Pulmonary-Renal SyndromesMcCall, A. ScottBhave, GautamPedchenko, VadimHess, JacobFree, MeghanLittle, Dustin J.Baker, Thomas P.Pendergraft, William F.Falk, Ronald J.Olson, Stephen W.Hudson, Billy G.2018-10-02T07:26:26-07:00doi:10.1681/ASN.2018050519hwp:resource-id:jnephrol;29/11/2619American Society of NephrologyCopyright © 2018 by the American Society of NephrologyJournal of the American Society of Nephrologyanti-GBM disease, ANCA, extracellular matrix, glomerulonephritis, Goodpasture-s syndrome, vasculitisRapid CommunicationRapid Communicationresearch-article20182018-11-01November 201810.1681/ASN.20180505191046-66731533-34502018-10-02T07:26:26-07:002018-11Journal of the American Society of NephrologyRapid Communication2911112619260526252607Background Complement-fixing antibodies against donor HLA are considered a contraindication for kidney transplant. A modification of the IgG single-antigen bead (SAB) assay allows detection of anti-HLA antibodies that bind C3d. Because early humoral graft rejection is considered to be complement mediated, this SAB-based technique may provide a valuable tool in the pretransplant risk stratification of kidney transplant recipients. Methods Previously, we established that pretransplant donor-specific anti-HLA antibodies (DSAs) are associated with increased risk for long-term graft failure in complement-dependent cytotoxicity crossmatch-negative transplants. In this study, we further characterized the DSA-positive serum samples using the C3d SAB assay. Results Among 567 pretransplant DSA-positive serum samples, 97 (17%) contained at least one C3d-fixing DSA, whereas 470 (83%) had non–C3d-fixing DSA. At 10 years after transplant, patients with C3d-fixing antibodies had a death-censored, covariate-adjusted graft survival of 60%, whereas patients with non–C3d-fixing DSA had a graft survival of 64% (hazard ratio, 1.02; 95% confidence interval, 0.70 to 1.48 for C3d-fixing DSA compared with non–C3d-fixing DSA; P=0.93). Patients without DSA had a 10-year graft survival of 78%. Conclusions The C3d-fixing ability of pretransplant DSA is not associated with increased risk for graft failure.10.1681/ASN.2018020205Thu, 26 Jul 2018 06:21:56 GMT-07:00Pretransplant C3d-Fixing Donor-Specific Anti-HLA Antibodies Are Not Associated with Increased Risk for Kidney Graft FailureBackground Complement-fixing antibodies against donor HLA are considered a contraindication for kidney transplant. A modification of the IgG single-antigen bead (SAB) assay allows detection of anti-HLA antibodies that bind C3d. Because early humoral graft rejection is considered to be complement mediated, this SAB-based technique may provide a valuable tool in the pretransplant risk stratification of kidney transplant recipients. Methods Previously, we established that pretransplant donor-specific anti-HLA antibodies (DSAs) are associated with increased risk for long-term graft failure in complement-dependent cytotoxicity crossmatch-negative transplants. In this study, we further characterized the DSA-positive serum samples using the C3d SAB assay. Results Among 567 pretransplant DSA-positive serum samples, 97 (17%) contained at least one C3d-fixing DSA, whereas 470 (83%) had non–C3d-fixing DSA. At 10 years after transplant, patients with C3d-fixing antibodies had a death-censored, covariate-adjusted graft survival of 60%, whereas patients with non–C3d-fixing DSA had a graft survival of 64% (hazard ratio, 1.02; 95% confidence interval, 0.70 to 1.48 for C3d-fixing DSA compared with non–C3d-fixing DSA; P=0.93). Patients without DSA had a 10-year graft survival of 78%. Conclusions The C3d-fixing ability of pretransplant DSA is not associated with increased risk for graft failure.Kamburova, Elena G.Wisse, Bram W.Joosten, IrmaAllebes, Wil A.van der Meer, ArnoldHilbrands, Luuk B.Baas, Marije C.Spierings, EricHack, Cornelis E.van Reekum, Franka E.van Zuilen, Arjan D.Verhaar, Marianne C.Bots, Michiel L.Drop, Adriaan C.A.D.Plaisier, LoesSeelen, Marc A.J.Sanders, Jan StephanHepkema, Bouke G.Lambeck, Annechien J.A.Bungener, Laura B.Roozendaal, CarolineTilanus, Marcel G.J.Voorter, Christina E.Wieten, Lottevan Duijnhoven, Elly M.Gelens, Mariëlle A.C.J.Christiaans, Maarten H.L.van Ittersum, Frans J.Nurmohamed, Shaikh A.Lardy, Neubury M.Swelsen, Wendyvan der Pant, Karlijn A.M.I.van der Weerd, Neelke C.ten Berge, Ineke J.M.Bemelman, Frederike J.Hoitsma, Andries J.van der Boog, Paul J.M.de Fijter, Johan W.Betjes, Michiel G.H.Heidt, SebastiaanRoelen, Dave L.Claas, Frans H.Otten, Henny G.2018-07-26T06:21:56-07:00doi:10.1681/ASN.2018020205hwp:resource-id:jnephrol;29/9/2279American Society of NephrologyCopyright © 2018 by the American Society of NephrologyJournal of the American Society of Nephrologychronic allograft failure, kidney transplantation, anti-HLA antibodies, complement-fixing antibodiesRapid CommunicationRapid Communicationresearch-article20182018-09-01September 201810.1681/ASN.20180202051046-66731533-34502018-07-26T06:21:56-07:002018-09Journal of the American Society of NephrologyRapid Communication29922792285Background Three different cell types constitute the glomerular filter: mesangial cells, endothelial cells, and podocytes. However, to what extent cellular heterogeneity exists within healthy glomerular cell populations remains unknown. Methods We used nanodroplet-based highly parallel transcriptional profiling to characterize the cellular content of purified wild-type mouse glomeruli. Results Unsupervised clustering of nearly 13,000 single-cell transcriptomes identified the three known glomerular cell types. We provide a comprehensive online atlas of gene expression in glomerular cells that can be queried and visualized using an interactive and freely available database. Novel marker genes for all glomerular cell types were identified and supported by immunohistochemistry images obtained from the Human Protein Atlas. Subclustering of endothelial cells revealed a subset of endothelium that expressed marker genes related to endothelial proliferation. By comparison, the podocyte population appeared more homogeneous but contained three smaller, previously unknown subpopulations. Conclusions Our study comprehensively characterized gene expression in individual glomerular cells and sets the stage for the dissection of glomerular function at the single-cell level in health and disease.10.1681/ASN.2018030238Thu, 24 May 2018 06:17:29 GMT-07:00A Single-Cell Transcriptome Atlas of the Mouse GlomerulusBackground Three different cell types constitute the glomerular filter: mesangial cells, endothelial cells, and podocytes. However, to what extent cellular heterogeneity exists within healthy glomerular cell populations remains unknown. Methods We used nanodroplet-based highly parallel transcriptional profiling to characterize the cellular content of purified wild-type mouse glomeruli. Results Unsupervised clustering of nearly 13,000 single-cell transcriptomes identified the three known glomerular cell types. We provide a comprehensive online atlas of gene expression in glomerular cells that can be queried and visualized using an interactive and freely available database. Novel marker genes for all glomerular cell types were identified and supported by immunohistochemistry images obtained from the Human Protein Atlas. Subclustering of endothelial cells revealed a subset of endothelium that expressed marker genes related to endothelial proliferation. By comparison, the podocyte population appeared more homogeneous but contained three smaller, previously unknown subpopulations. Conclusions Our study comprehensively characterized gene expression in individual glomerular cells and sets the stage for the dissection of glomerular function at the single-cell level in health and disease.Karaiskos, NikosRahmatollahi, MahdiehBoltengagen, AnastasiyaLiu, HaiyueHoehne, MartinRinschen, MarkusSchermer, BernhardBenzing, ThomasRajewsky, NikolausKocks, ChristineKann, MartinMüller, Roman-Ulrich2018-05-24T06:17:29-07:00doi:10.1681/ASN.2018030238hwp:resource-id:jnephrol;29/8/2060American Society of NephrologyCopyright © 2018 by the American Society of NephrologyJournal of the American Society of Nephrologyglomerulus, single-cell RNA sequencing, scRNAseq, podocyte, transcriptomeRapid CommunicationsRapid Communicationsresearch-article20182018-08-01August 201810.1681/ASN.20180302381046-66731533-34502018-05-24T06:17:29-07:002018-08Journal of the American Society of NephrologyRapid Communications29882060203620682038Background C3 glomerulopathy (C3G) is a life-threatening kidney disease caused by dysregulation of the alternative pathway of complement (AP) activation. No approved specific therapy is available for C3G, although an anti-C5 mAb has been used off-label in some patients with C3G, with mixed results. Thus, there is an unmet medical need to develop other inhibitors of complement for C3G. Methods We used a murine model of lethal C3G to test the potential efficacy of an Fc fusion protein of complement receptor of the Ig superfamily (CRIg-Fc) in the treatment of C3G. CRIg-Fc binds C3b and inhibits C3 and C5 convertases of the AP. Mice with mutations in the factor H and properdin genes (FHm/mP−/−) develop early-onset C3G, with AP consumption, high proteinuria, and lethal crescentic GN. Results Treatment of FHm/mP−/− mice with CRIg-Fc, but not a control IgG, inhibited AP activation and diminished the consumption of plasma C3, factor B, and C5. CRIg-Fc–treated FHm/mP−/− mice also had significantly improved survival and reduced proteinuria, hematuria, BUN, glomerular C3 fragment, C9 and fibrin deposition, and GN pathology scores. Conclusions Therapeutics developed on the basis of the mechanism of action of soluble CRIg may be effective for the treatment of C3G and should be explored clinically.10.1681/ASN.2018030270Tue, 12 Jun 2018 05:51:03 GMT-07:00Prevention of Fatal C3 Glomerulopathy by Recombinant Complement Receptor of the Ig SuperfamilyBackground C3 glomerulopathy (C3G) is a life-threatening kidney disease caused by dysregulation of the alternative pathway of complement (AP) activation. No approved specific therapy is available for C3G, although an anti-C5 mAb has been used off-label in some patients with C3G, with mixed results. Thus, there is an unmet medical need to develop other inhibitors of complement for C3G. Methods We used a murine model of lethal C3G to test the potential efficacy of an Fc fusion protein of complement receptor of the Ig superfamily (CRIg-Fc) in the treatment of C3G. CRIg-Fc binds C3b and inhibits C3 and C5 convertases of the AP. Mice with mutations in the factor H and properdin genes (FHm/mP−/−) develop early-onset C3G, with AP consumption, high proteinuria, and lethal crescentic GN. Results Treatment of FHm/mP−/− mice with CRIg-Fc, but not a control IgG, inhibited AP activation and diminished the consumption of plasma C3, factor B, and C5. CRIg-Fc–treated FHm/mP−/− mice also had significantly improved survival and reduced proteinuria, hematuria, BUN, glomerular C3 fragment, C9 and fibrin deposition, and GN pathology scores. Conclusions Therapeutics developed on the basis of the mechanism of action of soluble CRIg may be effective for the treatment of C3G and should be explored clinically.Wang, XiaoxuVan Lookeren Campagne, MennoKatschke, Kenneth J.Gullipalli, DamodarMiwa, TakashiUeda, YoshiyasuWang, YuanPalmer, MatthewXing, GuolanSong, Wen-Chao2018-06-12T05:51:03-07:00doi:10.1681/ASN.2018030270hwp:resource-id:jnephrol;29/8/2053American Society of NephrologyCopyright © 2018 by the American Society of NephrologyJournal of the American Society of Nephrologycomplement, glomerulopathy, membranoproliferative glomerulonephritis (MPGN)Rapid CommunicationsRapid Communicationsresearch-article20182018-08-01August 201810.1681/ASN.20180302701046-66731533-34502018-06-12T05:51:03-07:002018-08Journal of the American Society of NephrologyRapid Communications29882053203220592033Background Direct quantitative measurement of GFR (mGFR) remains a specialized task primarily performed in research settings. Multiple formulas for estimating GFR have been developed that use the readily available endogenous biomarkers creatinine and/or cystatin C. However, eGFR formulas have limitations, and an accurate mGFR is necessary in some clinical situations and for certain patient populations. We conducted a prospective, open-label study to evaluate a novel rapid technique for determining plasma volume and mGFR. Methods We developed a new exogenous biomarker, visible fluorescent injectate (VFI), consisting of a large 150-kD rhodamine derivative and small 5-kD fluorescein carboxymethylated dextrans. After a single intravenous injection of VFI, plasma volume and mGFR can be determined on the basis of the plasma pharmacokinetics of the rhodamine derivative and fluorescein carboxymethylated dextrans, respectively. In this study involving 32 adults with normal kidney function (n=16), CKD stage 3 (n=8), or CKD stage 4 (n=8), we compared VFI-based mGFR values with values obtained by measuring iohexol plasma disappearance. VFI-based mGFR required three 0.5-ml blood draws over 3 hours; iohexol-based mGFR required five samples taken over 6 hours. Eight healthy participants received repeat VFI injections at 24 hours. Results VFI-based mGFR values showed close linear correlation with the iohexol-based mGFR values in all participants. Injections were well tolerated, including when given on consecutive days. No serious adverse events were reported. VFI-based mGFR was highly reproducible. Conclusions The VFI-based approach allows for the rapid determination of mGFR at the bedside while maintaining patient safety and measurement accuracy and reproducibility.10.1681/ASN.2018020160Thu, 10 May 2018 08:01:14 GMT-07:00A Novel Method for Rapid Bedside Measurement of GFRBackground Direct quantitative measurement of GFR (mGFR) remains a specialized task primarily performed in research settings. Multiple formulas for estimating GFR have been developed that use the readily available endogenous biomarkers creatinine and/or cystatin C. However, eGFR formulas have limitations, and an accurate mGFR is necessary in some clinical situations and for certain patient populations. We conducted a prospective, open-label study to evaluate a novel rapid technique for determining plasma volume and mGFR. Methods We developed a new exogenous biomarker, visible fluorescent injectate (VFI), consisting of a large 150-kD rhodamine derivative and small 5-kD fluorescein carboxymethylated dextrans. After a single intravenous injection of VFI, plasma volume and mGFR can be determined on the basis of the plasma pharmacokinetics of the rhodamine derivative and fluorescein carboxymethylated dextrans, respectively. In this study involving 32 adults with normal kidney function (n=16), CKD stage 3 (n=8), or CKD stage 4 (n=8), we compared VFI-based mGFR values with values obtained by measuring iohexol plasma disappearance. VFI-based mGFR required three 0.5-ml blood draws over 3 hours; iohexol-based mGFR required five samples taken over 6 hours. Eight healthy participants received repeat VFI injections at 24 hours. Results VFI-based mGFR values showed close linear correlation with the iohexol-based mGFR values in all participants. Injections were well tolerated, including when given on consecutive days. No serious adverse events were reported. VFI-based mGFR was highly reproducible. Conclusions The VFI-based approach allows for the rapid determination of mGFR at the bedside while maintaining patient safety and measurement accuracy and reproducibility.Rizk, Dana V.Meier, DanielSandoval, Ruben M.Chacana, TeresaReilly, Erinn S.Seegmiller, Jesse C.DeNoia, EmmanuelStrickland, James S.Muldoon, JosephMolitoris, Bruce A.2018-05-10T08:01:14-07:00doi:10.1681/ASN.2018020160hwp:resource-id:jnephrol;29/6/1609American Society of NephrologyCopyright © 2018 by the American Society of NephrologyJournal of the American Society of NephrologyBiomarker, FAST BioMedical, acute kidney injury, chronic kidney disease, fluorescent glomerular filtration rate measurement, kidney functionRapid CommunicationRapid CommunicationRapid Communication20182018-06-01June 201810.1681/ASN.20180201601046-66731533-34502018-05-10T08:01:14-07:002018-06Journal of the American Society of NephrologyRapid Communication29616091613